![]() The relatively late developmental expression suggests a role for FGF-1 in neuronal maturation, rather than in neurogenesis. FGF-1B mRNA was expressed in phylogenetically older brain regions, which are involved primarily in processing information from exteroceptive sensory mechanoreceptors and in motor control. mesenchyme (mf: mesencephalic flexure, white outline) outside the neural tube (Ne) but absent in Iba1+ microglial progenitors within the neural tube. ![]() Although occasional brainstem nuclei were labeled at low levels by embryonic day 18, the majority of nuclei became detectable autoradiographically during postnatal weeks 1 and 2, and adult levels of grain density were reached during the 3rd and 4th postnatal weeks. In situ hybridization with cRNA probes specific for the 1B transcript showed the message to be restricted largely to sensory and motor nuclei in the brainstem, and to the ventral spinal cord and cerebellum. The sequences upstream from the major transcription initiation site lacked consensus TATA or CAAT boxes. An RNase protection assay was used to map the transcription start site of the 1B promoter. ![]() There are no defining structural changes, other than for the endoscopist trying to navigate the colon who may be able to see the dark extrinsic impression of the liver on the colon, thus marking the distinction between the end of the ascending and beginning of transverse colon. The mouse cDNA clone corresponded to the sole FGF-1 transcript in the brain. The hepatic flexure is the junction between the transverse colon and the ascending colon. Nucleotide sequence analysis revealed that the clone contained, in addition to the protein coding region, an untranslated exon (FGF-1B) 34 base pairs upstream of the translation start codon ATG. A cDNA clone coding for FGF-1 was isolated from a mouse brain cDNA library. The present study elucidates the molecular structure of a murine fibroblast growth factor 1 (FGF-1) promoter and describes its distribution in the adult and developing mouse brain. ![]()
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